Human Immunodeficiency Virus (HIV) causes Acquired Immunodeficiency Syndrome (AIDS). It is a serious disorder of the immune system in which the body’s normal defenses against infection break down, leaving it vulnerable to a host of life-threatening infections. Human immunodeficiency and Treponema pallidum causative organism of syphilis share mode of transmission and risk factors. The study was conducted to assess the extent of seropositivity of syphilis among HIV positive patients at tertiary care hospital in Rajkot from JUNE 2016 to MAY 2017 and evaluate the need of routine screening for this infection among such patients. The samples were tested for HIV as per Strategy III of National AIDS control organization by using different system of testing to establish diagnosis of HIV. Samples were tested for antibodies to Treponema Pallidum by qualitative Rapid Plasma Reagine (RPR); reactive samples were retested with quantitative RPR (to rule out biological false positive samples) and by qualitative Treponema Pallidum heamagglutination test (TPHA) for confirmation of positive samples. Out of total 300 HIV positive patients, 69.67% were male and 30.00% were female. Only one was transgender. Out of 300 samples tested, 7(2.33%) samples were positive for syphilis with 5(2.39%) of male and 2(2.22%) of female patients. Higher prevalence was observed in age group of 21-30yrs 3(4.62%) and in male patients. We believe our data could help health professionals to deal better with HIV infected patients. We also believe our data reinforces the need of prevention programs on HIV transmission, which also lead to reduction in prevalence of Syphilis.