Acinetobacter has emerged as significant hospital pathogen involved in outbreaks of hospital infections, notoriously known to acquire antibiotic resistance to most of the commonly prescribed antimicrobials. Many risk factors are associated with Acinetobacter infections, especially in patients in intensive care unit (ICU). Acinetobacter species tend to be resistant to a variety of antibiotics and thus the infections are difficult to treat. This study aims to isolate Acinetobacter from various clinical samples and to determine its antimicrobial sensitivity pattern. The objectives of the present study were; 1) To isolate Acinetobacter species from various clinical samples. 2) To study their Antibiogram pattern of the Isolated Organisms. A total of 93 Acinetobacter species were isolated from various clinical samples. Identification of Acinetobacter was done on the basis of hemolysis on blood agar, growth at 42ºC, and oxidation fermentation test. Antibiotic susceptibility testing was done as per standard CLSI guidelines (2017). Maximum isolation of Acinetobacter species were from pus or wound swabs 57 (61.29%) followed by sputum and tracheal secretions 21 (22.58%) and urine 15 (16.12%) samples. Most of the strains were sensitive to imipenem (97%), piperacillin-tazobactam (91.39%), and getifloxacin (86%) whereas, maximum resistance was observed to co-trimoxazole (10.75%) and gentamicin (9.67%). Acinetobacter spp. has emerged as a major nosocomial pathogen. Broad-spectrum antibiotics should be used with caution and only after antibiotic susceptibility testing. Early identification and continued surveillance of prevalent organism will help prevent the spread of Acinetobacter in hospital environment. Empirical antibiotic policy should be determined for each hospital according to the resistance rates of that hospital setting.