Chikungunya Fever is one of the most important arboviral infections of medical significance. It is characterized by an abrupt onset of fever with severe arthralgia followed by constitutional symptoms and rash lasting for 1-7 days. The disease is almost self-limiting and rarely fatal. Chikungunya virus (CHIKV) is a RNA virus belonging to the family Togaviridae, Genus Alpha virus. Aim of the study is to know the seroprevalence of Chikungunya infection in a Tertiary Care Hospital in Jamnagar, Gujarat, India. A retrospective study was conducted at a Microbiology Department, Shree M.P. Shah Govt. Medical College, Jamnagar, Gujarat. Serum samples were collected from 382 suspected cases of Chikungunya fever and tested for Chikungunya IgM antibodies by ELISA over a 1 year period from July 2017 to June 2018. Of the 382 serum samples tested, 67 (17.54%) were positive for Chikungunya IgM antibodies. Out of these 67 positive samples, males were 30 (44.78%) and females were 37 (55.22%). The most affected age group was 31 to 45 years 22 (32.84%), followed by 16 to 30 years 17 (25.36%). From the present study it can be concluded that the Chikungunya cases are on rise. Hence, Chikungunya has become a major public health problem in India. Favorable mosquitogenic condition during monsoon period is primarily responsible for the rapid spread of Chikungunya. This requires continuous monitoring of the viral circulation in both endemic and nonendemic areas and rapid implementation of Chikungunya control programme. For these infections early detection and access to proper medical care will cause lower fatality rate.