The study was conducted to assess the extent of seropositivity of Hepatitis C among HIV positive patients at tertiary care hospital in Rajkot from June 2016 to May 2017 and evaluate the need of routine screening for this infection among such patients. The samples were tested for HIV as per Strategy III of National AIDS control organization by using different system of testing to establish diagnosis of HIV. A total of 300 confirmed HIV-positive samples were tested for anti-HCV antibodies by using one step immunochromatographic assay based on the principle of the double antigen-sandwich technique. Out of total 300 HIV positive patients, 69.67% were male and 30.00% were female. Only one was transgender. Out of 300 samples tested, 5(1.67%) were positive for HCV with 2(0.96%) of male and 3(3.33%) of female patients. Highest prevalence was observed among age group 21–30yrs i.e 2(3.77%), while not a single anti-HCV positive case were recorded in age group ≤20 and ≥51 which implies that HIV and HCV infection is more common in younger generation. We believe our data could help health professionals to deal better with HIV infected patients. We also believe our data reinforces the need of prevention programs on HIV transmission, which also lead to reduction in prevalence of Hepatitis C.