Isolation of microorganisms is very important for accurate treatment and is one of the important concerns for clinical microbiologists. The accuracy and rapidity of treatment are critical for successful treatment. The present study was undertaken to find the time duration for detection of various microorganisms with BACTEC 9050 system. Methods: The present study was done on inpatients the Thumbay New Life Hospital, Chaderghat road, Hyderabad from the period of June 2016 to June 2017. Samples are drawn from the patient and injected directly into BACTEC 9050 culture vials. Positive cultures are immediately flagged by an indicator light on the front of the instrument and displayed on the monitor. When positive vials are identified, it is removed and isolated for identification of organisms. Results: Out of total 475 samples collected during this duration 315 samples were from male patients and 160 from female patients. Out of 315 male samples, 69 were found to have a positive culture in 69 (21.9%) samples. Similarly out of 160 samples from females 41(25.62%) were found to have a positive culture. The most common organism detected by BACTEC in positive cultures Enterobacteriaceae in 39 (35.45%) out of 110 samples Staphylococcus aureus in 21 (19.09%), Streptococcus spp in 15 (13.63%), CoagulaseNegative staphylococci in 13 (11.81%). The conventional method showed the growth only in 77 (16.21%) of the total 475 samples during the study period however the same samples in BACTEC showed 110 (23.15%) growth Average time to detection was highest in Cryptococcus spp in 78.9 hours, Corynebacterium spp 72.1 hours, Candida spp 65.5 hours. Least time to detection Enterobacteriaceae was 14.9 hours. Conclusion: it can be concluded that the BACTEC culture method is superior to the conventional method as it detects the presence of bacteria faster and it is not affected by patient's use of antibiotics and it is also economical. Hence BACTEC culture methods must be used whenever it is feasible.