EDTA-dependent pseudothrombocytopenia (PTCP) is the phenomenon of a low platelet count due to EDTA-induced aggregation of platelets. Since the failure to recognize EDTA-dependent PTCP may result in incorrect diagnosis and inappropriate treatment, the recognition of this phenomenon is very important in managing the patient and giving correct treatment. We here by discuss about a case of, A 21 year old male admitted for fever for evaluation showed severe thrombocytopenia by automated 5 part Mindray analyser. Manual count and peripheral smear done revealed higher count and small platelet aggregates. Following which citrated sample was collected and manual counting done both showed similar values, low count in singles without aggregation when compared to EDTA sample. EDTA induced platelet aggregation was thus conformed.