Introduction: Blood is an essential therapeutic need, as there is no genuine substitution. Blood transfusion saves many lives in cases of emergency, and is of more concern to transfuse safe blood to avoid further complications mainly transfusion-transmissible infections transfusion-transmissible infections (TTI‟s). In existence of World Health Organization (WHO) approved national hemovigilance protocols in different regions, blood transfusion carries a high risk for both patients and healthcare workers. Materials and Methods: This is a retrospective study was carried at Tertiary Care Teaching hospital on consecutive blood donors‟ records covering the period between September 2019 and August 2020. This blood bank is the center where an effective blood banking system is catering for all the blood needs of patients in the hospitals of this region. Blood samples were collected aseptically by venipuncture from the donors. The samples were analyzed for blood group, HIV-1 & 2, HBV, HCV, VDRL and Malaria following standard procedures. Result: In this study, of the 983 samples, 964 were males and 19 females, which correspond to 98.1% of male and the rest female. The maximum number of patients were in the age group of 18-30 years which were 69.8% (n =687) of total followed by age group 31–50 years having 30.0% (n = 30) in this group and 0.10% were 51-70 years. In this study, the most of donors had „O‟ Blood Group 48.7% (n =479) and least were AB Blood Group 6.5% (n = 64) in this study. The seroprevalence of HIV positive cases were 0.81%, HBV positive cases were 0.91%, HCV positive cases were 0.30%, VDRL positive cases were 0.71% and Malarial Parasite 2.3%. Conclusion: The low prevalence of HIV, HBV, HCV, VDRL and Malaria observed in present study may be due to awareness of donors, strict donor selection criteria and affective screening of the donors.