Staphylococcus aureus is one of the most common causes of Blood Stream infections (BSI), skin and wound infections, osteomyelitis, endocarditis, and nosocomial infections, especially pneumonia, surgical site infections (SSI), and continue to be a major cause of community-acquired infections. Methicillin Resistant Staphylococcus aureus (MRSA) is an important cause of community and hospital acquired infections. MRSA are mainly nosocomial and are increasingly reported from many countries worldwide. The purpose of present study was to determine the sensitivity of S. aureus isolated from infected patients to methicillin and to evaluate the possible presence of VRSA in our tertiary care hospital. Staphylococci were isolated and identified by standard microbiological procedures. Methicillin resistance was detected by using cefoxitin (30 µgm) by disc diffusion method. MRSA strains detected were then subjected to vancomycin agar screen test and E test to detect vancomycin resistance. Out of the total 500 S.aureus isolates, methicillin resistance was observed in 47.4% of isolates. . By E-test, 24.1 % MRSA isolates had Vancomycin MIC value of 0.75 and 21.9% of MRSA had Vancomycin MIC value of 0.5. Only one isolate had MIC value of 2.