Abstract: Several new biochemical and hematological parameters are available to diagnose and monitor alcoholic liver disease (ALD), but none are independently sufficient for the purpose. Serum enzymes - Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), Gamma glutamyl transferase (GGT) and AST:ALT ratio (De Ritis Ratio) are conventionally used as markers for diagnosis, treatment, prognosis and monitoring of ALD, based on their correlation with values before and after treatment or abstinence from alcohol intake. We wanted to establish the utility and affordability of estimation of these serum enzymes in ALD. To assess the levels and analyse the costs of estimation of conventional serum liver enzymes in ALD. After IEC clearance, this hospital based study was carried out on 30 male patients of diagnosed ALD and 30 normal control (non-alcoholic, healthy male) subjects between 18-65 years without history of liver disease, HIV, upper gastrointestinal bleeding, shock and/or ischemia to the liver, hepatotoxic medication use or exposure to high levels of environmental hepatotoxins, renal disorders, diabetes mellitus, hypertension. Serum AST, ALT, ALP, GGT levels were estimated using commercially available Roche diagnostic kits and fully automated biochemistry analyser (eCOBAS 6000 c501, Roche). Enzyme levels were expressed as Mean ± SD in IU/ml. Statistical analysis of differences in Means was done with Unpaired Student’s ‘t’ test, using GraphPad Quickcalcs online software, considering significance level as P < 0.05 at 95% confidence interval. Cost calculation of all the tests was done. Serum AST, ALT, ALP, GGT levels were highly significantly raised in ALD patients compared to healthy controls. De Ritis ratio was greater than 2 in 70% ALD patients. Cost calculation revealed that each patient had to pay a total of Rs 80/- for the tests. Conventional serum liver enzyme estimation and calculation of De Ritis ratio are useful and affordable diagnostic and prognostic markers in ALD.