Abstract: Main objective of this study is to utilize Agro wastes instead of pure sugars for cellulases productions. It was noted that maximum fungal cell growth was obtained 0.936g/100ml of Aspergillus niger was observed at pH 4.11 in hydrolyzed rice husk used as carbon source. Aspergillus niger was isolated from the soil of Khairpur. The collection and utilization of suitable Agro wastes used as a carbon source for cellulases production by fungi requirements optimized fermentation process. Five agricultural wastes were measured for cellulolytic enzyme production using pretreatment methods acid. Acid pretreatment was found to be the most efficient and best method for higher enzyme production. Using this cheap and renewable residue, for cellulolytic enzyme production by Aspergillus niger boosts its economic value which is not comparable with its current use as animal feed. Agro wastes such as sugarcane peelar bagasse, sugarcane bagasse, banana fruit stalk, sorghum husk and rice husk were hydrolyzed with 0.6N HCl. Rice husk was found good substrate in comparison to other Agro wastes for the growth of Aspergillus niger and cellulases production. Maximum activity of cellulases were noted 4.811 (units/ml). The Cellobiase and salicinase maximum production 4.717 and 4.742 units per ml obtained at 240 hours respectively.