Ecotoxicity strength of different granular domestic detergents on Enterobacter aerogenes and Pseudomonas fluorescens was evaluated to assess the response of the test organisms to different brands of domestic detergents. Standard toxicity procedures were applied using four brands of granular domestic detergents: Klin, Omo, Bonux and Ariel prepared at concentrations of 10ppm, 100ppm, 1000ppm, 10000ppm and 100000ppm; the test was carried out for 0h, 4h, 8h, 12h, and 24h for each detergent.The degree of resistance of the test organisms to the toxicants revealed Enterobacter aerogenes (4857±1685ppm) was more resistant to the domestic detergents than Pseudomonas fluorescens (117±28ppm). Mean Median lethal concentration (LC50) showed that Klin was most toxic and Bonux was least toxic with toxic flow pattern (noting that the lower the LC50 the more toxic the toxicant) as:Klin (1354ppm) > Omo (2389ppm) > Ariel (2887.47ppm) > Bonux (3316.84ppm).Results of response of individual test organism to the different brands of detergents showed that Pseudomonas fluorescens was most sensitive to Bonux and least sensitive to Ariel - Bonux (94.86ppm) > Klin (104.66ppm) > Omo (109.68ppm) > Ariel (157.40ppm))while Enterobacter aerogenes was most sensitive to Klin and least sensitive to Bonux - Klin (2603.34ppm)> Omo (4668.68ppm) > Ariel (5617.54ppm) > Bonux (6538.82ppm). This showed that test bacteria responded differently to the toxic effect of the domestic detergents in the same aquatic environment. In mixed consortium, domestic detergent Klin had the highest toxic strength (mean LC50 1354ppm); Pseudomonas fluorescens was more sensitive to all the test domestic detergents than Enterobacter aerogenes.