Introduction: Infection Control measures are based on how an infectious agent is transmitted and include standard, contact, droplet and airborne precaution. Infection Control is an important part in the dentistry. Provision of dental care is a risk of transmitting infectious diseases. The main aim of this study is to assess the knowledge and compliance of Dental health Care Providers on infection control standard precautions guidelines in Primary Health Care Centers in Makkah, Saudi Arabia. The output of this study is helpful for higher authorities to develop appropriate and sound policies to increase knowledge and compliance on this issue. The main objective of the study is to determine the level of compliance of Dental Health Care Providers on infection control standard precautions guidelines in Primary Health Care Centers in Makkah, Saudi Arabia, 2018. Methods: A questionnaire based cross sectional survey was conducted in dental clinics of 42 Primary Health Care Centers in 4 major sectors, Makkah, Saudi Arabia. A total of 82 Dental Health Care Providers were assessed for Compliance on infection control standard guidelines and the factors that can relate to it. Descriptive analysis was performed to determine mean and standard deviation for continuous variables and proportion for categorical variables. Bivariate analysis was performed to explore the relationship between compliance of Dental Health Care Providers on infection control standard precautions guidelines and knowledge of DHCPs on infection control standard precaution guidelines, age, gender, history of chronic diseases, smoking status, marital status, type of job and CBAHI accredited PHCCs were assessed by chi square test at a 95% confidence level and P-value ≤0.05 was taken as significant. Results: Descriptive statistics showed that the mean age of the DHCPs was 34 ± 5.05 SD. Majority (74.4%) were female providers. About 43.9 % of the DHCPs were dentist and 56.1 were dental assistants. This study showed that 78 % DHCPs classified as adequate knowledge. Among all DHCPs, 93.9 % aware about infection control standard precautions guideline while 56.1 % of the DHCPs received basic infection control skill license. Bivariate analysis showed that, among all DHCPs, 19.5 % classified as adequate compliance. There was no statistically significant difference between compliance of Dental Health Care Providers on infection control standard precautions guidelines and knowledge of DHCPs on infection control standard precaution guidelines, age, gender, history of chronic diseases, smoking status, marital status, type of job and CBAHI accredited PHCCs. Conclusion: This study showed very low compliance of Dental Health Care Providers on infection control standard precautions guidelines.