Non-fermenters (NFs) account for about 15% of all bacterial pathogens isolated from a clinical microbiology laboratory. Primarily regarded as contaminants, they have been implicated in causing various infections. They are innately resistant to many antibiotics and emerging as important nosocomial pathogens. To isolate nonfermenters from Urinary Tract Infections and assess their clinical significance and antimicrobial sensitivity pattern.Five thousand nine hundred and thirty four (5934) nonrepetitive consecutive samples of urine from patients diagnosed of culture-proven UTI of a tertiary-care teaching hospital over a period of four-years (2014-2017) were selected. A urinary sample was subjected to presumptive screening followed by semiquantitative culture on CLED (Himedia, Mumbai) agar. Identification and antibiogram was done by Vitek 2 compact (bioMérieux, France) automated systems. Clinical history and laboratory parameters were utilized for clinical correlation.Out of 5934 samples showing significant growth 6212 (15.6%) bacterial isolates were grown, Non fermenters being in 5.6%. P. aeruginosa (55.17%)and Burkholderia cepacia (17.42%) were predominant NFs.