The objective of the study was to evaluate the aphrodisiac property of the aqueous extract of Prunusafricana trunk bark in albino and normal male rats of the Wistar strain. Colorimetric and precipitation methods were used to highlight secondary metabolites of the plant. The acute toxicity test was conducted according to OCDE guideline 423. Four lots of 6 animals were used to evaluate the aphrodisiac property of P. africana. The positive control received sildenafil citrate (5 mg/Kg) and the negative control, distilled water. The test lots received the extract at 100 and 200 mg/Kg for 8 days. The copulatory parameters were observed on days 1, 4 and 8. The phytochemical screening revealed the presence of flavonoids, alkaloids, tannins, saponosides and tri terpenes in the extract. No changes in the general appearance of the rats and no mortality were recorded during the toxicity test, highlighting that P. africana is nontoxic. The extract significantly increased the sex parameters of the tested rats. This aphrodisiac effect of P. africana attributed to identify secondary metabolites justifies its use in traditional medicine as a sexual stimulant.