The educational environment is the general atmosphere of a learning space. A safe valued, participatory education environment supports autonomy and influences learning, achievement, and satisfaction. Education institutions aspire to provide a positive educational environment and quite often, seek feedback from learners to assess the quality of the education environment. Gender segregation is a norm in the Saudi Arabian education system. Educational institutions in Saudi Arabia do make efforts to establish a similar education environment across male and female campuses. Using a cross-sectional survey approach, learners' perceptions of the educational environment at one of the public Universities in the Asir region in Saudi Arabia were assessed with a purpose to generate a profile of the institution’s strengths and limitations. Dundee Ready Educational Environment Measure (DREEM), a generic tool was administered to learners enrolled in twelve undergraduate health professional programs, in the year 2017-2018. A total of 646 learners responded to the survey. The majority of the learners perceived the education environment as 'more positive than negative'. None of the items had a mean score of 3.5 and above, indicating 'no strengths'. Fourteen 'problem areas' were identified. Gender differences in the perceived education environment were noted in the Emergency Medical Services (EMS), Physiotherapy, and Doctor of Pharmacy programs. Male learners perceived the educational environment across programs alike. However, female learners did not. DREEM was useful to identify the priorities in planning appropriate measures to improve the education environment.