Isolation of fungal species from unused toilet papers is essential, to decipher the public health risk associated with the exposure of these sanitary papers in public toilets, prior to use by students. Unused tissue papers kept at public toilets were sampled from eleven (11) different locations (labelled F1-F11) to cover the various faculties in the University. The fungal counts and characterisation was done following standard microbiological procedures. The result revealed that Total Fungal Count ranged between 3.5±0.4 x 104 cfu/g, (obtained from the Faculty of Humanity toilet rooms) and 1.3±0.2 x 104 cfu/g, obtained from Faculty of Engineering toilet rooms. Phenotypic characterisation of the fungal isolates revealed the isolates obtained from the various samples included; Aspergillus niger, Aspergillus fumigatus, Aspergillus flavus, Candida species and Penicillium species. The percentage occurrence of the isolates showed Aspergillus niger as the dominant species (38%), while Candida species was the least occurring (17.2%). The study has shown that exposure of unused tissue papers, prior to use in toilets may pose severe health risk to the students. Tissue papers should therefore be kept in sterile packs prior to use in public toilets.