The composition and therapeutic values of the essential oils obtained from the leaves of O. gratissimum and L. martinicensis was investigated. The GC-MS results reveals the presence of bioactive compound such as eucalyptol (10.99%), estragole (48.52%) and linalool (24.49%) in O. gratissimum while for the Leucas martinicensis its reveals the presence of diazoprogesterone (0.15%), Isoaromadendrene epoxide (0.10%) and beta.-copaene (4.37%). Oxalic acid, oleic acid, p-Menth-8(10)-en-9-ol, and Caryophyllene was detected in the essential oils of both plants. The IR-spectroscopy results confirmed the functional group of most of the compound identified. Test for tannins, flavonoids, steroids, cardiac glycoside, alkaloids terpenoid, and anthraquinone were positive in methanolic and aqueous extracts of both plants. The in vitro antimicrobial screening of the essential oils against S. aureous, E.coli, S. pneumonia, K. pneumonia, A. niger and C. albicans showed that they are potential antimicrobial agents. The DPPH scavenging activity of essential oils showed that L. martinicensis with an IC50 of (145.7ug/ml) have higher antioxidant activity than essential oil obtained from the leaves of O. gratissimum with the IC50 of (158.3ug/ml) but their activities is lower than the standard with IC50 of (134.3ug/ml) and (145.7ug/ml) respectively.