Introduction: Hypertension is one of the important causes of coronary heart disease and cerebrovascular disease. The autonomic nervous system has a significant role in the circulatory system and blood pressure regulation. Analysis of heart rate variability (HRV) is a non-invasive tool that is widely used to assess autonomic nervous function. Aims & Objectives: The study aimed to analyze HRV in Hypertensive individuals. Materials & Methods: An analytical study was conducted on 50 normotensives and 50 hypertensives in the age group of 40–60 years. Blood pressure was measured by using a sphygmomanometer in sitting position. According to JNC VII criteria, normotensives are those with SBP ≤ 120 mmHg and DBP ≤ 80 mmHg. Hypertensives are those with SBP ≥ 140 mmHg and DBP ≥ 90 mmHg. HRV is measured for 2min using Polar H10 chest strap and data is analyzed using Elite HRV software. In Time-domain methods Mean RR, SDNN, RMSSD, and PNN50 were assessed. In Frequency domain methods LF, HF, and LF/HF were assessed. The data was entered into Excel 2016 and SPSS version 21 was used for analysis. An unpaired “t” test was used to compare the HRV values between normotensive and hypertensive groups. Results: Mean HR, SDNN, RMSSD, PNN50, and HF were found to be decreased and LF, LF/HF were increased in hypertensives suggesting increased sympathetic activity and reduced parasympathetic activity in hypertensives. All the values were statistically significant (p<0.01). Conclusion: There is increased sympathetic activity and a decreased vagal tone associated with hypertension. Thus HRV can be used as a routine screening test to predict the future risk of hypertension at an earlier stage and also for a better prognosis during treatment.