Pseudomonas aeruginosa is a true opportunistic pathogen and is responsible for causing a variety of infections in clinical settings in both immunocompetent as well as immunocompromised hosts. However, in most cases, P.aeruginosa infections occur in patients who have been compromised in some way, e.g. chronic Diabetic Patients. Methods: In this study a total of 280 (10.98%) P.aeruginosa isolates were obtained out of 2549 samples collected for a period of 5 years. The isolates were selected on the basis of their growth characteristics on Blood agar, MacConkey agar and Nutrient agar medium. Colonies were subjected to series of biochemical tests to identify the species. Antimicrobial susceptibility testing of all the confirmed P.aeruginosa isolates was performed by automated walkaway 96 using NBC 42 and conventionally comparing with Kirby–Bauer disc diffusion method for further confirmation and results were interpreted according to CLSIs guidelines. Results: The prevalence of this pathogen was 10.98% and most of the isolates were mostly isolated from pus/wound swab cultures, followed by urine & sputum cultures, mostly isolated from critical areas such as ICU’s.