Endoscopy with endoscopic biopsy is currently the major method of diagnosis of gastrointestinal (GI) neoplasms. A total of 60 endoscopic biopsy specimens from upper and lower gastrointestinal tract were studied in the laboratory from November 2013 to October 2014 in the department of pathology, Acharya Shree Chander College of Medical Sciences and Hospital (ASCOMS), Jammu. Of these gastrointestinal biopsies 25 (41.66%) were from upper gastrointestinal tract and 35 (58.33%) were from the lower gastrointestinal tract. Among these upper and lower GI biopsies a total of 35 (58.33%) suspected neoplastic lesions were included in the present study. The biopsies that were included in the study comprised of 6 (10%) esophageal biopsies, 12 (20 %) gastric biopsies, 3 (5%) from GEJ, 4 (6.66%) from duodenum, 2(3.33%) from pyloro-duodenum, 1 (1.66%) from jejunum, 2 (3.33%) from ileum, 14 (23.24%) from colon, 12 (20%) from rectum, 4 (15%) from anus. There are only few studies on histopathological spectrum on GI endoscopic biopsies in Jammu region. ASCOMS is a tertiary care hospital where patients come from all over Jammu region. Analysis of biopsied material therefore can provide a fairly good estimate of spectrum of various gastrointestinal lesions (neoplastic/non-neoplastic/pre-neoplastic) in Jammu region as well as comparison of our results with similar studies in other institutions.