Background: BAL specimens are widely used for diagnosing respiratory pathologies like chronic diseases, lung carcinomas, pneumonias, etc. Infections cause exacerbations. Study of bacterial profile, antibacterial sensitivity patterns of samples guides in timely & appropriate use of antibiotics, preventing emergence of antibiotic resistance. Aim: This study was done to isolate causative bacteria from BAL specimens to know their antibacterial sensitivity pattern. Settings and Design: A hospital-based prospective study was done on 118 BAL samples received in Microbiology laboratory from May 2019-July 2021 from wards for patients with respiratory pathologies. Material and Methods: Samples collected using proper aseptic precautions & sent, within 2 hours of collection were processed using standard procedures for bacterial isolation and species identification and antibiotic sensitivity testing done & reports made as per latest CLSI guidelines. Result: 15.25% samples showed growth. Klebsiella (65.21%), Pseudomonas (30.43%) and Acinetobacter (4.34%) were isolated. Klebsiella species isolated showed 95%, 97% and 98% sensitivity respectively to Cephalosporins, quinolones, aminoglycosides & 100% sensitivity towards higher antibiotics like Carbapenems, Piperacillin/Tazobactam, Tetracycline. An Extended spectrum ß lactamase producing Klebsiella species was isolated. Pseudomonas aeruginosa showed 80%, 85%, 90% and 95% respectively sensitivity to Ceftazidime; Gentamicin, Tobramycin; Amikacin, & 100% to Polymyxins. An isolate of Acinetobacter lwoffii was obtained. It was resistant to Ceftazidime. Conclusion: Knowledge of bacteriological and antibacterial profile of BAL samples helps in judicious use of antibiotics, preventing resistance & also in making local antibiogram to guide empirical therapy.