The present study aimed to evaluate the influence of light emitting diode (LED) on in vitro rooting of Moringa oleifera Lam shoots. To achieve this, the vitroplants were grown in MS mod 3B basal medium, supplemented with 20 g/l sucrose and without phytohormones, and subjected to white and red LED light for 4 weeks. At the end of the experiment, it was observed that there were significant differences between the LED lights used in terms of the number of roots and the weight of the callus obtained. The white LED gave vitroplants with many roots and reduced callus. Thus, the basic medium MS mod 3B, with 20 g.L-1 sucrose and without phytohormones, and the white LED could be used for a good rooting of M. oleifera in vitro plants.