Background: Genital Tuberculosis is one of the leading causes of infertility in Bangladesh which is a high tuberculosis burden country. As the patients are mostly asymptomatic and diagnostic tests have low sensitivity and specificity, diagnosis of genital tuberculosis is very much challenging. The aim of this study is to evaluate the patients of genital tuberculosis diagnosed at laparoscopy. Methods: A retrospective study was performed on 12 patients who underwent laparoscopy and hysteroscopy. Cases were analyzed according to history, relevant pre surgery investigation findings, laparoscopic and hysteroscopic findings, histopathology, MTB PCR and Acid-Fast Bacillus (AFB) tests reports. Results: Mean age 25 years, range (22 -33), ten (83.3%) with primary subfertility and two (16.6%) with secondary subfertility. Two had secondary amenorrhea, two women presented with scanty menstrual flow and seven women with regular cycle with average flow and duration. Two (16.6%) had previously completed treatment for pulmonary tuberculosis, 4(33.3%) had history of exposure to tuberculosis patient. Hysterosalpingography showed eleven (91.6%) cases with bilateral tubal block, one (8.3%) with only left tubal block. At laparoscopy all women had tubal involvement, ovarian involvement in 6(50%), frozen pelvis or dense pelvic adhesion in one. At hysteroscopy 11 (91.6%) cases had intrauterine adhesion. Histopathology of endometrial tissue revealed tubercular granuloma in two (16.6%) cases; MTB PCR was detected in 6(50%) cases and acid-fast bacillus was negative in all cases. Conclusions: Laparoscopic and hysteroscopic findings are more suggestive than history, microscopic, histopathological and molecular examination of endometrial tissue in diagnosing genital tuberculosis in infertile women.