Ethnomedicine is currently practiced in Egypt, where has been documented by ethnobotanical surveys. The main objective of the current study is to evaluate phytochemistry and antibacterial potential of ethanolic and aqueous extracts of P. juliflora cultivated in Matruh region with a view to considering their contribution to human health. Qualitative and quantitative phytochemicals screening of pods and leaves was carried out. Five pathogenic bacteria were selected as recipients including four Gram-negative and one Gram-positive bacteria. Chocolate and blood agar solid media were applied for agar diffusion method. Extracellular proteins before and after treatment were measured and total protein assay was performed. Cytological effect of the ethanolic extracts was investigated via TEM. Phytochemical screening provides the occurrence of alkaloids, cardiac glycosides, coumarins, flavonoids, phenolics, steroids, tannins and terpenes in pods and leaves ethanolic and aqueous extract. In agar diffusion experiment, data showed that pod ethanolic extract (5%) was more active than the other three types of extracts. The extract elicited a decrease in the growth of Staphylococcus aureus in culture medium in comparison to the control. Both pods and leaves ethanolic extracts showed a decrease in the extracellular protein content of the tested S. aureus compared with control. TEM showed that pod ethanolic extract affected the growth of S. aureus cells. It caused severe damage in the cytoplasmic membrane and also the cell wall. The present study concluded that the pods and leaves extracts of P. juliflora possesses antibacterial potential against target bacterial type cultures.