The exploration of antibiotic and antiseptic resistance in the hospitals are happened by various reasons including continuous usage of same antiseptics and disinfectants for surface cleaning, inappropriate and misuse of antimicrobials, No follow up of the patients and discontinuation of the course of drugs. This may create a demand for new and long term effective drugs against antibiotic resistance. Comparing to the chemicaly fabricated drugs, metals are considered as the better choice of replacement. From the ancient times, the basic understanding of antimicrobial properties of metal particles like silver, mercury, copper and arsenic are quite available in practice; but their scientific evidences are lacking. Thus this study may provide some information related to the determination of in vitro antibacterial activity of various concentrations of silver solution against several bacterial pathogens isolated from wounds. The evident record of this study highlighted that 10-2 mM silver solution showed maximum bactericidal effect on Staphylococcus aureus, Providencia rettigeri and Pseudomonas aeruginosa with 17mm, 16mm and 16mm inhibitory zone respectively. Other test bacterial species also inhibited but not upto the mark. We may recommend the high concentration of silver solution in the form of antiseptic spray as surface bactericidal mask for wound management.