The hypomineralized enamel lesions have 20% less mineral content, substantially reduced calcium and phosphorus ratio, and an increased carbon content which increases the likelihood of demineralization. The application of remineralizing agents on these lesions have been tried. In the present pilot study, to assess the change in mean fluorescence after the application of self-assembling peptide (SAP) on hypomineralized enamel lesions on ten MIH-affected incisors using laser fluorescence. After a single application of SAP, there was a decrease in the mean fluorescence score record with Diagnodent at two-time intervals i.e., baseline and one-month post-application. Thus, it can be concluded that the SAP application could use as a viable treatment option.