Arbuscular Mycorrhizal Fungi (AMF) is a type of mycorrhizal that is in symbiosis with most plants. AMF symbiotic association provides many benefits for plants such as to help the nutrient absorption, especially phosphor, survival in drought conditions. Bacteria that can increase the growth and development of mycorrhizae is known as Mycorrhiza Helper Bacteria (MHB). The aim of pot experiment was to analyze the quality of the inoculum on mycorrhizal propagation enriched with the addition of MHB. The experiment used a Randomized Block Design to test three treatments of MHB liquid formula in the AMF propagation. The MHB liquid formula consisted of Pseudomonas diminuta, Bacillus subtilis, and a mixture of both isolates and controls (sterilized distilled water). The results revealed that mycorrhizal propagation using MHB increased the colonization of maize root and population of P. diminuta and B. subtilis in the mycorrhizal inoculum. Significant sporulation of Glomus spp. and bacterial population were increased by MHB inculation were also observed.