Background: Adolescence is the most critical phase of human growth that radically alters the physiology of the body and wherein any inconsistency can lead to serious health consequences in adulthood. In adolescents, the haematological and biochemical parameters change with age, necessitating a continuous age-related definition of the reference intervals. This prospective study aimed to examine the utility of new haematological and biochemical markers to assess nutritional status and possible health risks in children, adolescents and adult boys. Materials and Methods: We performed a cross-sectional study in a sizeable group of 90 male participants aged between 10–22 years to inspect the distribution of values of common haematological and biochemical parameters. Anthropometrical measurements were carried with standard protocols, and blood samples were collected from participants in a hospital setting. Haematological samples were measured using pathology laboratory protocols. Hepatic, renal, lipid and carbohydrate metabolism markers were determined by following standard biochemistry methods. Results: We observed some differences between haematological parameters, hepatic, renal, lipid and glycaemic profiles among children, adolescents and adults. Amongst lipid parameters, only HDL levels were significantly associated with gender following puberty (p< 0.001), the calculated BMI levels were in the lower normal range. Conclusions: This is the first study establishing haematological and biochemical parameters in this part of India. These findings provide a helpful guide for clinical researchers and care providers. Studies on a large scale and in different settings would also be desirable.