Introduction: Wound infection remains an important cause of morbidity and mortality among humans, especially in developing countries. Knowledge regarding Bacterial isolates and their antibiotic susceptibility pattern in pus isolate is necessary for empirical treatment of wound infections and useful for making antibiotic policy of hospital. Aim: The present study was conducted to assess bacterial isolates and their antimicrobial susceptibility patterns from pus samples of Sir T Hospital, Bhavnagar. Material and method: The present study was carried out during July 2019 to November 2019 in Microbiology Department, Sir T Hospital, and Bhavnagar. The pus samples received in bacteriology section of microbiology laboratory were preceded for bacterial identification and antibiotic susceptibility testing. It was done by Modified kirbybauer disk diffusion method according to CLSI guidelines 2019. Result: A total of 1110 pus samples were received from which 477 (42.97%) samples were positive for gram positive 117(24.52%) and gram negative 360 (75.47%) bacteria. About 117(24.52%) of the total isolates were Staphylococcus aureus, 220 Escherichia coli (41%), 96 Klebsiellaspp (17%), 16 Pseudomonas (13%), 13 Proteus mirabilis (2%), 11 Proteus vulgaris (2%), 4 Acinatobacter species (0.74%). Gram negative organisms were sensitive for Meropenem (98%), Piperacillin Tazobactem (77%) Gram positive organism were completely sensitive to Vancomycin (100%), Linezolid (100%). Conclusion: Thus the present study shows that Escherichia coli, Klebsiellaspp, Pseudomonas and Staphylococcus aureus are the most common bacteria showing sensitivity towards vancomycin, linezolid, meropenem, piperacillin tazobactum. This study helps in decidiing proper treatment of wound infection.