A multi-location experiment was conducted at three locations of New Halfa irrigated scheme, Hajer, the Faculty and Elshebaik sites, for two consecutive seasons 2017/2018 and 2018/2019 to test the effect of 18 organo-chemical fertilizer levels on growth attributes, yield and yield components of two wheat genotypes Bohain and Debaira. The results of analyzed data showed that. In both seasons, the growth attributes, such as the plant height, number of leaves per plant, leaf area (cm2) showed non-significant differences for both genotypes under the different 18 fertilizer levels at Hajer and the Faculty sites. However, these traits are significantly different (P≤ 0.05) for both genotypes at Elshebaik site. Contrary to the growth attributes, the effect of the organo-chemical fertilizer was highly significant (P≤0.001) for the yield and most of its components for both genotypes in both seasons and at the three sites of study. The yield of both genotypes at the three locations is better in the first season compared to the second one. Debaira yield for both seasons and at the three locations is better than the yield of Bohain. From this study, it is clear that the yield of both genotypes increased with addition of the organic manure. From the results obtained in this study, it could be concluded that, Debaira scored better yield per h (kg) compared to Bohain genotype in both seasons and at two locations, Chicken manure up to the rate of 4 ton /h. when combined with 43kg N/h and 43kg P/h improved the yield of both genotypes in both seasons and at the three locations.