Introduction: Infection with the metallo-beta-lactamase (MBL) producing organisms is associated with higher rates of mortality, morbidity, and health care costs. MBL producing Pseudomonas aeruginosa was first reported in Japan in 1991 and since then has been found in various parts of the world. Objective: To Assess the Dissemination of Class 1 integron among different MBL producing Acinetobacter baumannii In ICU of DMCH, Dhaka, Bangladesh. Methodology: This Cross sectional study was conducted between July 2013 to June 2014 in the department of Microbiology, Dhaka Medical College (DMC), Dhaka, Bangladesh to determine the frequency and susceptibility patterns of MBL-producers among carbapenem-resistant Gram-negative rods (GNRs) from clinical isolates of a tertiary care hospital. All clinical samples were processed according to standard microbiological methods. Isolated GNRs were subjected to susceptibility testing against various antibiotics by disc diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Carbapenem-resistant isolates were subjected to the detection of MBL production by the E-test MBL strip method. Hospitalized patients who did not give consent were excluded in this study. Data regarding age, sex, duration of hospitalization, antibiotic history were collected using a prescribed data collection form. Results: Out of 22 imipenem resistant Acinetobacter baumannii. 21 (95.45%) were positive for MBL production. The distribution of MBL genes among imipenem resistant Acinetobacter baumannii. Out of 22 imipenem resistant strains, 21 (95.45%) were positive for blaNDM-1, 20 (90.91%) for blaNDM-like, 16 (72.72%) for blaVIM and all the 22 (100%) were negative for blaIMP and distribution of class I integron among the MBL producers. Among the 21 MBL producing Acinetobacter baumannii, 17 (80.95%) had class I integron. This study presence of class I integron and conserved segment of class 1 integron among the different MBL producing Acinetobacter baumannii. Among 16 blaVIM positive Acinetobacter baumannii. 13 (81.25%) carried class 1 integron and 11 (68.75%) carried conserved segment of class I integron. Out of 21 blaNDM-1 positive strains. 17 (80.95%) carried class I integron and 13 (61.9%) carried conserved segment of class 1 integron. Out of 20 blaNDM-like positive strains, 16 (80%) carried class 1 integron and 12 (60%) carried conserved segment of class I integron. Conclusion: The findings strongly suggest that there is a need to track the detection of MBL producers and that judicious use of imipenem is necessary to prevent the further spread of these organisms.