Background: Prevalence of anxiety and other mental disorders have increased in medical health care workers during the Covid-19 Pandemic. Aim: To assess the anxiety among health care professionals during sampling for COVID-19 and its assessment via RT-PCR technique. Material and methods: A questionnaire based online survey was carried out on 134 health care workers including Dentists, Doctors and Nursing officers. The Questionnaire had demographic details and a set of 11 questions that analyzed the anxiety during various phases of sampling. Results: Out of 134 participants, 61.9% were males and 38.1% were females. The mean age of the participants was¬¬¬¬ 25.34 years. The anxiety among the health care professionals while undergoing sampling for covid-19 via. RT-PCR technique was found to be high. Conclusion: The anxiety among the health care professionals undergoing sampling for Covid-19 with RT-PCR technique was high considering the invasiveness of the nasal, the fear of getting a positive result for COVID and fear of acquiring infection from personnel/patients in the waiting hall. Post vaccination trials can possibly help in evaluating the change in anxiety among the various Health Care Workers (HCW’S) on receiving the COVID report.