Background: Increased workload and safety concerns have major psychological effects on healthcare personnel. Since the introduction of vaccination, there is some improvement in transmission and severity of Covid-19 symptoms. Methods: This was a cross-sectional study which was done at Security Forces Hospital, Riyadh, and Kingdom of Saudi Arabia. The objective of this study was to examine the effect of vaccination or being vaccinated on stress among nurses working in a tertiary care hospital. We calculated the sample size as 68. We used the Perceived stress scale (PSS) in our study. We gave the questionnaire comprising PSS questions to nurses from different wards. We calculated the mean, standard deviation, frequency count, and percentages for the perceived stress. Results: The mean PSS-10 score was 17.52 (SD: 6.40) with a range between 0 and 31. Thirteen (19.4 %) nurses had low stress and 51 (76.11 %) nurses had moderate stress while 3 (4.4 %) nurses had high stress. Conclusion: Vaccination against Covid-19 has made significant improvement in stress level among nurses during current pandemic.