Objectives: The aim of this study was to investigate the concentration of hemoglobin, ferritin, albumin, total protein and globulin in pregnant Sudanese women to assess the presence of anemia and hypoproteinemia in the second and third trimester. Materials and method: A total of 100 pregnant women were attended at Almadina Arab Hospital in Gezira state, Sudan in the period from December 2017 to February 2018. Their ages ranged from 19 to 37 years old, and they were divided into two groups: second trimester and third trimester. Venous blood samples were drawn from each participant to determine hemoglobin, ferritin, albumin, globulin, and total protein levels using a Spectrophotometric method by an automated analyzer (Biosystem A25), and globulin was determined by calculation. The statistical package for social science (SPSS) software version 20 was used to analyze data. Result: In both the second and third trimesters, the mean concentration of measured biochemical parameters were decreased, with no significant difference between the two groups however, the means of ferritin levels were normal. Hemoglobin mean levels were (10.784±1.143 and 10.968±1.570), total protein mean levels were (6.632±0.701 and 6.644±0.631), albumin mean levels were (3.204±0.423 and 3.254±0.420), and globulin mean levels were (3.444±0.661 and 3.390±0.611) in the second and third trimesters, respectively. The mean levels of ferritin were (36.900±1.458 and 39.300±1.591) in the second and third trimesters, respectively. Conclusion: Pregnant Sudanese women in their second and third trimesters were found to have anemia and hypoprotienemia.