Dengue virus (DENV) is the most common mosquito-transmitted human flavivirus worldwide, and it is well-established that DENV and Zika virus are closely related, resulting in substantial antigenic overlap. The infection is caused by any of the four dengue virus (DENV) serotypes transmitted by Aedes aegypti and albopictus mosquitoes. Despite the public health relevance, data on the prevalence of DENV infections among febrile patients in Jos, North Central Nigeria are limited. We aimed to determine the sero-prevalence of Dengue virus infection among febrile patients. Cross sectional study was conducted among 118 participants presenting with pyrexia at the Jos University Teaching Hospital. Participants who were malaria negative using rapid diagnostic tests (mRDT) with specific symptoms defined as probably dengue by WHO (fever and symptoms such as headache, rash, nausea/vomiting, joint pain, fatigue, retro-ocular pain and haemorrhage) were screened for dengue immunoglobulin G and M (IgG and IgM) using Dengue NS1 Antigen and IgG/IgM antibody duo panel RapiCardTM InstaTest and ELISA-based kit.