Background: Mullerian agenesis also known as Mayer- Rokitansky- Kuster- Hauser Syndrome is a congenital disorder characterized by agenesis of the uterus and upper part of vagina. Vagina is created or lengthened by non-surgical vaginal dilatation or surgical vaginoplasty to allow sexual function.We have been doing non-surgical vaginal dilatation facilitated initially by saline injection and sustained digital pressure in women with Mullerian agenesis for a few years. This was a follow up study with the purpose to evaluate the sexual function of these women. Method: We practiced a novel approach to quicken non- surgical dilatation of vagina. We interviewed women who received this treatment over cell phone after two months to four years. Result: A total fifteen women who had nonsurgical vaginal dilatation in our department were called over cellphone. Total 10 women could be reached.The age range of women was between 16 and 35 years. The frequency of intercourse was 3 to 5 times per week in couples living together. All eight women admitted of the experience of full genital performance during sexual intercourse and said they could provide pleasure to their partners. When asked about the presence of stable relationships and a good communication with the partners only five out of eight agreed. The reason behind strained relationship with husband was not sexual dysfunction but inability to conceive. Conclusion: Short term procedure of saline injection and sustained digital pressure under supervision can augment vaginal dilatation with vaginal dilators and lead to adequate sexual activity.