Plant growth promoting microorganisms (PGPM) are the rhizosphere bacteria and Vesicular Arbuscular Mycorrhizal spores (VAM) that enhance plant growth and suppress plant diseases.The current study was carried out at Ahmed Orabi Agricultural Cooperative Association, Ismailia, Egypt, to investigate the efficient antagonistic bacterial isolates and VAM against Fusarium wilt disease in pepper plant under filed experiment. A total of 49 bacterial isolates were isolated, out of which 4 (P1, P11, P19 and P42) were selected based on their antagonism of phytopathogens. Phylogenetic analysis of 16SrRNA sequences identified these isolates as new strains of Bacillus subtilis, Bacillus aerius, Achromobacter xylosoxidans and Lysinibacillus fusiformis. The selected isolates produced high levels siderophore and hydrogen cyanide. Disease symptoms, disease index percent, phytochemicals and Metabolic indicators of resistance in plant as response to induction of systemic resistance (SR) in pepper plants were recorded. VAM and P42 were the best isolates and reduced percent disease indexes by 10 and 15 % respectively. P11, and PGPR (P1, P11, P19, P42) gave the same result 17.5%. and came next P19, (VAM+PGPR) and P1 which recorded 20,22.5and 30 % respectively. The beneficial effects of the used elicitors were extended to increase not only total phenol, total soluble protein content but also the activities of peroxidase and polyphenoloxidase enzymes in comparison with control. At the same time, the results appeared that pepper infected plants treated with applied elicitors showed variation in number, molecular weight of protein bands as well as variability in number of polypeptide peroxidase and polyphenol oxidase isozymes in the leaves.