Background: Metabolic syndrome is common in women with polycystic ovary syndrome. Some studies have suggested that vitamin D deficiency may play role in metabolic disturbance and vitamin D supplementation improves metabolic profile of these patients. Objective: To evaluate the effect of vitamin D supplementation on the metabolic profile in the polycystic ovary syndrome patients. Methods: Seventy seven diagnosed cases of vitamin D deficient polycystic ovary syndrome patient with metabolic syndrome, age range 18-45 years were administered standard therapy of oral vitamin D (OH)2 40000 IU weekly for seven weeks followed by maintenance therapy of oral vitamin D2 2000 IU daily. Follow up vitamin D was done 3 months after initiation of vitamin D supplementation. The changes in features of metabolic syndrome namely, waist circumference, blood pressure, fasting plasma glucose, fasting serum triglyceride and high density lipoprotein levels were recorded. Paired‘t’ test was done to see if the changes were significant. Result: Mean serum vitamin D was significantly increased (11.5±4.0 ng/ml vs. 36.8±10.7 ng/ml) after the vitamin D supplementation (p<0.05). Triglycerides level decreased (180.7±62.3 vs 148.1±46.2 mg/dl) and HDL level increased (38.2±11.3 vs 47.1±10.3 mg/dl) which were statistically significant (p<0.05). But the changes were not significant for the waist circumference (93.9±8.6 vs. 93.4±8.2), blood pressure (SBP-117.6±12.4 vs 117.5±11.9 mm Hg; DBP- 80.9±7.6 vs 80.8±7.8 mmHg), fasting glucose (5.77±1.02 mmol/L vs. 5.74±0.99 mmol/L). Conclusion: Vitamin D supplementation have favorable effects on features of metabolic syndrome.