Advances in molecular biology techniques have been made due to biomedical engineering approaches for synthesis of new machineries and biomaterials. There are different molecular techniques that have been used for detection of microbes in order to distinguish their cells on the basis of their characteristics and structural differences of the same group’s organism. These molecular techniques are PCR, electrophoresis, DNA fingerprinting, Pulse electrophoresis. Besides these techniques for detection of DNA, PCR demand has become most during pandemic of Covid-19. Real time PCR is the most advanced form of PCR that detection the nucleic acid testing in short time with greater chances of accuracy as compared to the other kit based traditional methods for detection of nuclear segments. Loop-mediated isothermal amplification (LAMP) is used for viral detection through nucleic acid testing with high accuracy and has become popular technique during pandemic of Covid-19. Bacteria can be utilized for human benefits especially for industrial applications such Viruses infect all types of life forms, from animals and plants to microorganisms, including bacteria. As treatment of many diseases, food industry, pharmaceutical industries and medical industry.