Nanotechnology playing important role for the synthesis of chemical compounds by emphasizes on their applications in different fields due to their physical and chemical properties. ICP-OES is the most advanced technique with modifications in their instrumental working leads to effective analysis of chemicals compounds, nanoparticles characterization, trace elements in water and other geographical applications. Failure to achieve the calibration leads to ineffective characterization through ICP-OES that especially functioned in high recoveries of Au NPs when using such a special macerating enzyme that appeared to release the NPs from plant tissue without changing the size distribution of the NPs. ICP-OES is used for capturing the toxins and heavy metals in environment as the biological pollutants. Its efficiency to remove the nearby metals and other portents deliberately increased due to advances in the fields of the biomedical engineering in conjugation with the environment sciences. ICP-OES is widely used in mining processes, mining purity control, rocks analysis, etc.