The drinking water (potable water) is that water which is safe for human consumption .The analysis of drinking water determines the identification of bacteria and the effect of some other metals on them. The work was carried out in Sri Ganganagar zone. The samples were collected from 15 to 35 km. area of Sri Ganganagar,Rajasthan. The work was completed in the department of microbiology of Tantia University, Sri Ganganagar. Isolation and cultivation of Escherichia coli (E.coli) and Staphylococcus aureus(S.aureus) was done by using varies isolation techniques like serial dilution method, spreading etc. and applied different media like nutrient agar, Eosin Methylene Blue , MacConkey agar and special media and various biochemical reactions. Therefore, S.aureus and E.coli strains were selected for this antibacterial study. Both gram positive and gram negative microorganisms were used for the test and Al, Zn, Mn, and Fe metals were selected for study of antimicrobial effects. Prepared the cultured plate of E.coli and S.aureus and add the metals in the middle of the Petri plate and after overnight incubation obsevered the effect of metals. The result shown that Mn has no antimicrobial effect but Al, Fe and Zn has antimicrobial properties. Fe shown strong effect as compared to Al and Zn. The object of this study to investigate the antimicrobial effect of metals such as Mn, Al, Zn and Fe on drinking water bacteria such as E.coli and S.aureus. Antimicrobial properties of metals will be use for purification of drinking water and remove the bacterial contamination from the drinking water with the help of metals.