Objective of this study was to determine Biochemical condition and Microbiological load of water and soil sample of some costal shrimp Hatchery and Fisheries of Bangladesh. 8 samples were taken from 6 individual costal shrimp Hatchery. pH, temperature, Dissolved Oxygen, Biological Oxygen Demand, Ammonia, Salinity and Alkalinity of each sample was determined. The temperature and pH of the collected samples were found to vary from 29 to 32°C and 7.5 to 8.5 respectively. The Biological Oxygen Demand of the collected samples from different sources was found to range from 8.0 to 13.0 mg/L. The Dissolved Oxygen of the samples was observed to vary from 3.0 to 4.0 ppm. The ammonia content was observed to vary from 0.5 to 0.8 mg/L. The salinity was in the range of 28 to 32 ppt. The alkalinity of the samples was found to range from 120 to 130 mg/L. The total bacterial count and Vibrio load count of was done from collected samples. The maximum bacterial load was found to exist in the soil sample of dumping ground and at the zone of mixing point of hatchery discharged water with sea water next to it.