Objectives: Using the method of meta-analysis to compares and analyzes the development characteristics of children’s FMS in countries with different development levels from the perspective of gender. Methods: We searched the PubMed, EBSCOhost, Embase and Web of Science databases for studies published up to April 5, 2021, empirical studies on the FMS of children aged 3-6 were included. Two researchers independently screened and extracted data according to inclusion and exclusion criteria. RevMan 5.4.1 software was used for meta-analysis to analyze the mastery level of global children’s FMS. Results: Finally, 18 articles were included. Results of the meta-analysis showed that whether in developed or developing countries, there was no statistical significance in comparing the locomotor skill scores of boys and girls (all P > 0.05); however, both in developed countries and developing countries, the scores of object control skills generally appears that boys are better than girls (Developed: 0.48 [95% CI 0.40, 0.56], Z=11.74, P＜0.05, I2=0%; Developing: 0.53 [95% CI 0.30, 0.76], Z=4.53, P＜0.05, I2=81%). Conclusion: From the perspective of gender, the developmental characteristics of FMS in children showed consistent patterns in countries with different development levels. There is no difference in the mastery of locomotor skills among children of different genders, but there is a significant difference in the mastery of object control skills, and boys are significantly better than girls.