Background: Apheresis is a blood transfusion process that allows a donor to give specific blood components like platelets, RBCs, or plasma to the receivers. During this procedure, the rest blood components are returned to the donors. For safe and effective apheresis platelet transfusion, profiling of donors and receivers or primary information of them is very important. In Bangladesh, we have not enough research-based information regarding these issues. Aim of the study: The aim of this study was to analyze the profile of apheresis platelet donors and receivers who were cancer patients. Methods: This was a retrospective observational study which was conducted in the Department of Transfusion Medicine, National Institute of Cancer Research and Hospital (NICRH), Mohakhali, Dhaka, Bangladesh during the period from January 2018 to December 2019. In total 210 platelet donors and 210 platelet receivers through apheresis procedure were enrolled as the study population. The ethical committee of the mentioned hospital had approved this study. Before starting data collection, properly written consent was taken from all the participants. All data were processed, analyzed, and disseminated by MS Office and SPSS version 20 as per need. Result: In this study, the mean age of the donor was 26.62 ± 5.86 years. Only one donor was female (0.5%) whereas 95.5% of donors were male. As platelet count, below 2 lacs, above 2 lacs, above 3 lacs, and above 4 lacs were in 10%, 72.4%, 16.7%, and 1% donors respectively. For 100% donors, it was a single donation. On the other hand, the mean age of the receivers was 26.94 ± 15.55 years. We observed, 69% of receivers were male whereas 31% were female. In this study, as platelet count of receivers, we found <10,000/lac, 10,000-30,000/lac and 30,001-50,000/lac platelets among 15.7%, 61.9% and 22.4% receivers respectively. The majority of the receivers were with AML (Acute Myeloid Leukemia) which was found among 68%. As a total number of apheresis, a single number was found in 13% and multiple numbers were found in 36% of receivers. In 82% of receivers, history of chemotherapy and in 18% receiver’s history of chemotherapy as well as radiotherapy were found. Conclusion: In Bangladesh basically males are the prominent part of platelet donation through apheresis procedure. Although young males play a vital role in apheresis platelet donation, younger children and adolescents are the basic receivers here, a large number of healthy young women as potential blood donors are still out of platelet donation procedure in Bangladesh. Necessary motivational education could make them interested as platelet donors in the mainstream blood transmission system in near future.