Antimicrobial resistance became a growing public health problem in the world and sexually transmitted infections (STIs) are one of the five types of pathologies which special care should be given. Staphylococcus aureus appear the most prevalent bacteria encountered causing infections in male urethral discharge (MUD) at Cotonou in Benin. It prevalence and it resistance to antibiotics were evaluated. This paper aims to describe the bacterial profile and antimicrobial susceptibility of S aureus strains isolated from MUD specimens at the National Laboratory (NL) of Heath Ministry. A retrospective study included 81 men at Cotonou suspected MUD during 1st January 2004 and 31st December 2015. MUD swabs were collected and analyzed to identify the bacteria by standard biochemical reactions, screening of trichomonads and detection of intracellular Gram-negative diplococci within phagocytes. The three main pathogens encountered among 81 MUD were Staphylococcus aureus (30/81; 37.04%), Staphylococcus spp (19/81; 23.46%) and Staphylococcus dore (12/81; 14.81%). S aureus susceptibility ranged from 60- 80% for minocyclin, lincomycin, chloramphenicol and gentamycin. But resistance was observed to ofloxacin, kanamycin, amoxicillin, amoxicillin-clavulanic acid, oxacillin, ceftriaxon, tetracyclin, trimethoprim+Sulfonamides and ampicillin between 59-100%. Conclusion: This study highlights the importance of routine susceptibility tests to antibiotics in the treatment of S aureus responsible of MUD in Benin.