Diabetes mellitus (DM) is one of the main problems in health systems and a global public health threat that has increased dramatically over the past two decades. According to epidemiological studies, the number of patients with DM increased from about 30 million cases in 1985, 177 million in 2000, 285 million in 2010, and estimated if the situation continues, to be more than 360 million people by 2030. Too many of the nearly 300 million people in the world with diabetes suffer from diabetes related foot complications. The understanding of the bacteriology of the diabetic septic foot is critical for guiding antibiotic selection and correlating culture results with appropriate therapy. This study is aimed to investigate the phenotypic and genotypic resistance among bacteria causing Diabetic Septic Foot patients. Bacterial isolates of diabetic patients with septic foot infection attending Jaber Abolez Diabetic Center from the 1st of February till the 31st of March, 2017 were recruited to the study. Total number of 204 wound swabs has been cultured during the study. Gram negative bacilli predominated as 183 isolates and Gram positive cocci as 30 isolates only. The commonest organism isolated was Proteus mirabilis as 96 isolates (45.07%). Regarding resistance; All Staphylococci identified are resistant to Oxacillin, resistance to Ciprofluxacin, Gentamicin and Amikacin is shown to be 25.7%, 50% and 27.9%, respectively. BlaCTX-M gene was detected in 27 multi-drug resistant Gram negative isolates (64.29%). The preponderance of ESBLs among the isolates from diabetic foot infections as well as tremendous drug resistance is of concern. Further research is needed to support these findings.