Objectives- The burden of diphtheria is more than what it seems to be, so early diagnosis and appropriate treatment is needed to prevent catastrophe. The study is aimed to stipulate the importance of microbiological diagnosis in cases of diphtheria. Thus, we report some confirmed cases from this region. Materials and Methods- 17 clinically suspected cases were studied from October 2020 to February 2021. Two throat swabs were collected. One swab was used for making two direct smears which were subjected to Gram stain and Albert stain respectively. Another swab was inoculated on Nutrient agar, Mac-conkey agar, Blood agar, Chocolate agar and Potassium tellurite blood agar (PTBA). Growths were observed after 48hrs of incubation at 37 o C aerobically. Colonies isolated from PTBA were subjected to Gram stain and Albert’s stain. Results- 6 out of the 17 cases showed positive growth on PTBA. Macroscopically grey-black opaque colonies were obtained. Microscopic examination of the Gram-stained smears showed gram positive, pleomorphic, club shaped bacilli with L and V patterns. Albert’s stain showed green colored bacilli with bluish-black polar metachromatic granules. Conclusion- Awareness among people, widespread universal immunization coverage, availability of modern microbiological support and rapid early diagnosis are the cardinal steps to control diphtheria resurgence.