Organizational culture refers to the unique relationship of norms, values, beliefs and ways of behaving that characterize how groups and individuals get things done. Organizational culture contains values that must be understood, imbued, and practiced together by all individuals or groups involved in it, including employees within the Regional Secretariat of Hulu Sungai Utara Regency. This study aims to determine how much influence organizational culture, work discipline and perceived organizational support influence on employee performance at the Regional Secretariat of Hulu Sungai Utara Regency, either partially or simultaneously. This study uses a quantitative approach with the type of explanatory research. The type of research used is explanatory research and the method used in this research is causal associative research. This research was conducted at the Regional Secretariat of Hulu Sungai Utara Regency, South Kalimantan and the research population is all employees who are still actively working at the Hulu Sungai Utara Regency Regional Secretariat with the status of Civil Servants (PNS) taken from the number of PNS in January 2020, which is 99 people. The research sample used in this study was a saturated sample or total sampling, or the entire population including the research sample, namely the entire 99 civil servants. The results showed that the positive constant value was 38.950, meaning that although there was no independent variable (X), the employee's performance would be better because it was supported by responsibility in carrying out their duties as regional employees working at the Hulu Sungai Utara Regional Secretariat. Organizational culture variable (X1) has a partially positive effect of 0.242 with t count 7.146 > t table 1.653 and significant level 0.000 <0.05. The work discipline variable (X2) has a partially positive effect of 0.326 with t count 5.331 > t table 1.653 with a significant level of 0.000 <0.05, and the perceived organizational support variable (X3) has a partial positive effect of 0.326 with t count 6.724 > t table 1,653 with a significant level of 0.000 <0.05. From these results it can be seen that all independent variables (X) have a simultaneous influence on the independent variable (Y) with an R2 value (Regression Square) of 0.594 or 59.4% contributing to the influence of organizational culture, work discipline and perceptions of organizational support. On the performance of employees at the Hulu Sungai Utara Regional Secretariat. So that the hypothesis Ha is accepted and H0 is rejected, which means that there is a simultaneous positive influence between organizational culture, work discipline and perceptions of organizational support on employee performance at the Hulu Sungai Utara Regional Secretariat.