This is the first study of aeromycoflora from the two places Ad Darb and Shuqaiq in the Jizan province. The diversity of aeromycoflora of the two places was isolated to know the environmental status. A total number of sixteen outdoor and indoor environmental samples of aeromycoflora were collected and studied from the two places along with the College of Science and Arts for Girls at Ad Darb. The aeromycoflora was cultured by open petri plate method during the month of April 2017 . A qualitative and quantitative assessment of the indoor and outdoor mycoflora was carried out by exposing the Potato Dextrose Agar petri plates in the different environments each for a period of two minutes.The fungal cultures on the petri plates and the slides were identified by microscopic and macroscopic characteristics. The outdoor aeromycoflora was represented by Aspergillus niger, Aspergillus flavus, Aureobasidium, Curvularia, Dresclera, Epicoccum, Fusarium, Penicillium and Yeast. The indoor aeromycoflora was represented by Aspergillus, Colletotrichum , Penicillium, Chrysosporium and Ulocladium. The most predominant genus was Aspergillus. The colony forming unit (CFU)3 of each of the represented mycoflora was calculated to find out the level of contamination at the different sites. The (CFU)3 values indicated that the outdoor mycoflora was higher than the indoor mycoflora .The fungal genera isolated are pathogenic. Hence a polluted aerial environment is harmful not only to human health but also to the animals and plant life .Therefore a constant monitoring for a clean and germ free environment is needed at all times.