This study was design to examine the microbial load, phytochemical screenings and acute toxicity of GM Plus herbal drug mixture. Six samples of the mixture were sent to the Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana for analysis. For the toxicity test, Sprague-Dawley rats were put into six rats in each group. Animals in all groups were immunized with 5.0*108 SRBC/ml after which each group was treated with either GM Plus (30,100 or 300 mg/kg p.o., daily) based on preliminary investigations, levamisole (10mg/kg, p.o, daily), dexamethasone sodium (4mg/kg, i.m, daily) or Normal saline. The result of the toxicity proved that, Levamisole and 30,100, and 300 mg/kg GM Plus treatment resulted in significant increases (p<0.001) in the HA titre and WBC count relative to the dexamethasone and no treatment groups. A differential count performed indicated an increase in neutrophil proportion of the total count in the GM Plus treated groups. With regards to the microbial analysis, the total microbial load of Gm Plus was within the acceptable limits (BP 2015; category C of herbal products) there were no pathogenic microorganisms present. The herbal mixture has complied with the BP specifications for microbiological quality. The product GM Plus is therefore safe for usage.